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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
From 2010.igem.org
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
| - | === | + | ===5:PCR=== |
<Member><br /> | <Member><br /> | ||
| - | + | nito | |
<Sample><br /> | <Sample><br /> | ||
| - | * | + | *vector |
| - | * | + | *4-5 |
| - | *3 | + | *1-2-3 |
| - | * | + | *10-2-3 |
| - | + | *7-8-10 | |
| - | + | *10-2-9 | |
| - | *7 | + | |
| - | * | + | |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===7：DNA extraction from gels=== | ===7：DNA extraction from gels=== | ||
Revision as of 11:00, 16 October 2010
Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
- 1-2-7
- 1-2
- 10-2-7
- 9-7
- 4-5
<Protocol>
See Protocol 8
<Result>
2：DNA extraction
＜Member＞
nito
＜Sample＞
- 1-2-7
- 10-2-7
- 9-7
＜Protocol＞
See Protocol 4
3：DNA Ligation
＜Member＞
nito
＜Sample＞
- 4-5
- 1-2-3
- 10-2-3
- 10-2-9
- 7-8-10
(after digestion)
＜Protocol＞
See Protocol 10
4：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
5:PCR
<Member>
nito
<Sample>
- vector
- 4-5
- 1-2-3
- 10-2-3
- 7-8-10
- 10-2-9
<Protocol>
See Protocol 1
7：DNA extraction from gels
＜Member＞
＜Sample＞
- 1+2(8/31)
- 4+5(8/31)
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
7：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- 1
- 2
- 3
- 4
- 6
- 10
・Digest enzyme
- AvrⅡ
- NheⅠ
＜Protocol＞
See Prptpcpl 9
1:PCR
<Member>
mio
<Sample>
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
<Protocol>
See Protocol 1
7：DNA extraction from gels
＜Member＞
＜Sample＞
- 1+2(8/31)
- 4+5(8/31)
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
3:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
