Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

From 2010.igem.org

(Difference between revisions)
(New page: =2010/10/14= ===3:Electrophoresis=== <Member><br /> mio,mizuki <Sample><br /> PCR products (9/16) <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Pro...)
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===3:Electrophoresis===
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===1:Electrophoresis===
<Member><br />
<Member><br />
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mio,mizuki
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nito
<Sample><br />
<Sample><br />
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PCR products (9/16)
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*1-2-7
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*1-2
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*10-2-7
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*9-7
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*4-5
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<Protocol><br />
<Protocol><br />
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<Result><br />
<Result><br />
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[[Image:Matsuura_2010-09-16_18hr_19min.JPG]]
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[[Image:]]
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===4:DNA extraction===
===4:DNA extraction===

Revision as of 10:52, 16 October 2010

Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

  • 1-2-7
  • 1-2
  • 10-2-7
  • 9-7
  • 4-5


<Protocol>
See Protocol 8


<Result>
[[Image:]]

4:DNA extraction

<Member>
mio,mizuki,nito

<Sample>
PCR products

  • 1
  • 3
  • 4
  • 5
  • 8
  • 10
  • 4-5
  • 7-8-10
  • 2
  • 6

Digestion products

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 7-8-10

<Protocol>
See Protocol 4


5:DNA Ligation

<Member>
mio,mizuki

<Sample>

  • 1-2
  • 4-5
  • 6A-7
  • 7-8-10

(after digestion)

<Protocol>
See Protocol 10


7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • 1
  • 2
  • 3
  • 4
  • 6
  • 10

・Digest enzyme

  • AvrⅡ
  • NheⅠ

<Protocol>
See Prptpcpl 9


1:PCR

<Member>
mio

<Sample>

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8


<Protocol>
See Protocol 1


7:DNA extraction from gels

<Member>


<Sample>

  • 1+2(8/31)
  • 4+5(8/31)

(After electrophoresis)


<Protocol>
SeeProtocol 4


7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • 1
  • 2
  • 3
  • 4
  • 6
  • 10

・Digest enzyme

  • AvrⅡ
  • NheⅠ

<Protocol>
See Prptpcpl 9


1:PCR

<Member>
mio

<Sample>

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8


<Protocol>
See Protocol 1


7:DNA extraction from gels

<Member>


<Sample>

  • 1+2(8/31)
  • 4+5(8/31)

(After electrophoresis)


<Protocol>
SeeProtocol 4


3:Electrophoresis

<Member>
mio,mizuki

<Sample>
PCR products (9/16)

<Protocol>
See Protocol 8


<Result>
Matsuura 2010-09-16 18hr 19min.JPG