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Team:Panama/20 July 2010
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Activities: | Activities: | ||
| - | 1.Locate the BioBrick parts that we are going to | + | 1.Locate the BioBrick parts that we are going to assemble, in the distribution plates. |
RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N | RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N | ||
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| + | '''Methodology Steps:''' | ||
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| + | For that we take the DNA from each of the wells of the plate. We then have to take 2ul of each one and put it in the bacteria. The other 8ul we are to store at -20°C | ||
2.Clone the chosen BioBrick parts in competent ''E. coli''. | 2.Clone the chosen BioBrick parts in competent ''E. coli''. | ||
Revision as of 20:24, 15 October 2010
July 20
Activities:
1.Locate the BioBrick parts that we are going to assemble, in the distribution plates.
RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N
Methodology Steps:
For that we take the DNA from each of the wells of the plate. We then have to take 2ul of each one and put it in the bacteria. The other 8ul we are to store at -20°C
2.Clone the chosen BioBrick parts in competent E. coli.
For the extraction we follow the comercial protocol.
We transformed 4 different bacteria, for the different BioBrick plasmids & prepared LB-ampi.
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