Team:TU Delft/Project/alkane-degradation/results

From 2010.igem.org

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This shows two things.
This shows two things.
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That E.Coli is partly able to degrade Dodecanol on it's own. And that this activity is greatly increased (38.4%) with the addition of ADH.
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That E.Coli is partly able to degrade Dodecanol on it's own. And that this activity is greatly increased (43.83% normalized for the amount of protein) with the addition of the ADH gene.
-
It is also clear that eventhough ADH is from a thermophilic organism it loses most of it's activity after heating.
+
It is also clear that eventhough ADH is from a thermophilic organism it loses most (63.5%) of it's activity after heating. the raw data for these calculations can be seen below.
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(Although this activity could also be attributed to the host organism lacking the proper post translational modifications.)
+
(Although this decrease in activity could also be attributed to the host organism lacking the proper post translational modifications.)
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 +
 
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
 +
|Conditions
 +
|'''Native'''
 +
|'''Native'''
 +
|'''Heated'''
 +
|'''Heated'''
 +
|-
 +
|Sample
 +
|J13002
 +
|K398018
 +
|J13002
 +
|K398018
 +
|-
 +
|Total protein in well
 +
|3.303E-02
 +
|3.180E-02
 +
|7.351E-03
 +
|7.994E-03
 +
|-
 +
|Specific activity (kat/mg)
 +
|1.812E-11
 +
|2.606E-11
 +
|0.000E-00
 +
|5.041E-12
 +
|}
===ALDH===
===ALDH===

Revision as of 16:15, 15 October 2010

Figure 1 – Schematic description of the alkane degradation pathway with the corresponding genes.


Contents

Alkb2 Cluster

LadA

ADH

ADH was tested with Dodecanol (C12)

This yielded the following results:

Figure 1 – The results of a resting cell assay done on biobrick number k398018.

This shows two things. That E.Coli is partly able to degrade Dodecanol on it's own. And that this activity is greatly increased (43.83% normalized for the amount of protein) with the addition of the ADH gene. It is also clear that eventhough ADH is from a thermophilic organism it loses most (63.5%) of it's activity after heating. the raw data for these calculations can be seen below. (Although this decrease in activity could also be attributed to the host organism lacking the proper post translational modifications.)


Conditions Native Native Heated Heated
Sample J13002 K398018 J13002 K398018
Total protein in well 3.303E-02 3.180E-02 7.351E-03 7.994E-03
Specific activity (kat/mg) 1.812E-11 2.606E-11 0.000E-00 5.041E-12

ALDH

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