Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/11
From 2010.igem.org
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[[Image:Matsuura 2010-10-11 01hr 51min.JPG]] | [[Image:Matsuura 2010-10-11 01hr 51min.JPG]] | ||
+ | |||
+ | |||
+ | |||
+ | ===PCR=== | ||
+ | <Member> | ||
+ | nito | ||
+ | |||
+ | |||
+ | |||
+ | <Sample> | ||
+ | |||
+ | *9 | ||
+ | *1+2 | ||
+ | *10+2 | ||
+ | *2 | ||
+ | *vector | ||
+ | |||
+ | |||
+ | <Protocol> | ||
+ | |||
+ | See Protocol2 | ||
+ | (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/) | ||
+ | |||
+ | |||
+ | *Tube(temperature in annealing) | ||
+ | |||
+ | :1.(60.0,50.0℃) | ||
+ | :2.(55.0℃) | ||
+ | :3.(55.0℃) | ||
+ | :4.(55.0℃) | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | ===Electrophoresis=== | ||
+ | |||
+ | <Member> | ||
+ | nito | ||
+ | |||
+ | |||
+ | |||
+ | <Sample> | ||
+ | |||
+ | PCR products. | ||
+ | :*9H | ||
+ | :*9L | ||
+ | :*1-2 | ||
+ | :*10-2 | ||
+ | :*2 | ||
+ | :*vector | ||
+ | |||
+ | |||
+ | |||
+ | <Protocol> | ||
+ | |||
+ | See Protocol8 | ||
+ | |||
+ | (https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/) | ||
+ | |||
+ | |||
+ | |||
+ | <Result> | ||
+ | |||
+ | [[Image:]] |
Revision as of 06:58, 15 October 2010
Contents |
2010/10/11
Electrophoresis
<Member>
nito
<Sample>
veutor+7-8-10
<Protocol>
See Protocol8
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
PCR
<Member> nito
<Sample>
- 9
- 1+2
- 10+2
- 2
- vector
<Protocol>
See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
- Tube(temperature in annealing)
- 1.(60.0,50.0℃)
- 2.(55.0℃)
- 3.(55.0℃)
- 4.(55.0℃)
Electrophoresis
<Member> nito
<Sample>
PCR products.
- 9H
- 9L
- 1-2
- 10-2
- 2
- vector
<Protocol>
See Protocol8
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
<Result>
[[Image:]]