Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/11

From 2010.igem.org

(Difference between revisions)
(Electrophoresis)
(2010/10/11)
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<Result><br />
<Result><br />
[[Image:Matsuura 2010-10-11 01hr 51min.JPG]]
[[Image:Matsuura 2010-10-11 01hr 51min.JPG]]
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===PCR===
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<Member>
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nito
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 +
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<Sample>
 +
 +
*9
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*1+2
 +
*10+2
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*2
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*vector
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 +
 +
<Protocol>
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 +
See Protocol2
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(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
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 +
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*Tube(temperature in annealing)
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:1.(60.0,50.0℃)
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:2.(55.0℃)
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:3.(55.0℃)
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:4.(55.0℃)
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 +
 +
 +
 +
 +
===Electrophoresis===
 +
 +
<Member>
 +
nito
 +
 +
 +
 +
<Sample>
 +
 +
PCR products.
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:*9H
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:*9L
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:*1-2
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:*10-2
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:*2
 +
:*vector
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 +
 +
 +
<Protocol>
 +
 +
See Protocol8
 +
 +
(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
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 +
 +
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<Result>
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 +
[[Image:]]

Revision as of 06:58, 15 October 2010

Contents

2010/10/11

Electrophoresis

<Member>

nito


<Sample>

veutor+7-8-10


<Protocol>

See Protocol8

(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)


<Result>
Matsuura 2010-10-11 01hr 51min.JPG


PCR

<Member> nito


<Sample>

  • 9
  • 1+2
  • 10+2
  • 2
  • vector


<Protocol>

See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)


  • Tube(temperature in annealing)
1.(60.0,50.0℃)
2.(55.0℃)
3.(55.0℃)
4.(55.0℃)



Electrophoresis

<Member> nito


<Sample>

PCR products.

  • 9H
  • 9L
  • 1-2
  • 10-2
  • 2
  • vector


<Protocol>

See Protocol8

(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)


<Result>

[[Image:]]