"
Team:HokkaidoU Japan/Notebook/September7
From 2010.igem.org
(Difference between revisions)
(→Checked concentration of DNA used yestrday for ligation) |
(→Ethanol percipication) |
||
| Line 5: | Line 5: | ||
* pSB1C3 band was very week | * pSB1C3 band was very week | ||
| - | + | =Ethanol precipitation== | |
In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done. | In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done. | ||
Revision as of 02:01, 15 October 2010
since 13 Jul 2010
since 1 Aug 2010
Checked concentration of DNA used yesterday for ligation
- 4 ng/uL RFP digested using either H buffer and M buffer
- pSB1C3 band was very week
Ethanol precipitation=
In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done.
Colony PCR of competent cells
Previous colony PCR produced strange bands so checked if there were from DNA of competent cell
- Colony PCR didn`t produce any bands so those bands had to be an artifact from plasmid used for transformation
