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Team:Panama/24 August 2010
From 2010.igem.org
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We performed the E. coli transformation, with plasmid (chloramphenicol resistance) + RBS + promoter. | We performed the E. coli transformation, with plasmid (chloramphenicol resistance) + RBS + promoter. | ||
This time we used NEB protocol. | This time we used NEB protocol. | ||
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1. Hind III marker (1 uL ladder, 2 uL loading, 7 uL H2O). | 1. Hind III marker (1 uL ladder, 2 uL loading, 7 uL H2O). | ||
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2. Sample (5 uL ligation + 2 uL loading). | 2. Sample (5 uL ligation + 2 uL loading). | ||
Revision as of 19:14, 14 October 2010
August 24
We performed the E. coli transformation, with plasmid (chloramphenicol resistance) + RBS + promoter. This time we used NEB protocol.
We made a miniprep and ran in agarose gel 1%.
1. Hind III marker (1 uL ladder, 2 uL loading, 7 uL H2O).
2. Sample (5 uL ligation + 2 uL loading).
