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Team:Stockholm/8 October 2010
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Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week. | Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week. | ||
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| + | ===Removal of insertion in BioBrick suffixes=== | ||
| + | ====Plasmid prep==== | ||
| + | Spun down pSB1C3.SOD ON culture at 13,000 x ''g'', 10 min. Supernatant discarded and cell pellet stored in -20 °C for later plasmid prep. | ||
Revision as of 11:45, 14 October 2010
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Andreas
Growth curve assay
Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks.
Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week.
Removal of insertion in BioBrick suffixes
Plasmid prep
Spun down pSB1C3.SOD ON culture at 13,000 x g, 10 min. Supernatant discarded and cell pellet stored in -20 °C for later plasmid prep.
