"
Team:Calgary/17 June 2010
From 2010.igem.org
(Difference between revisions)
(New page: '''Thursday June 17, 2010''' Dev, Chris, Jeremy: Today, Jeremy restreaked the construct of B0015-R0040, did a PCR on it. He then ran a gel electrophoresis on the five colonies that grew. ...) |
|||
| Line 1: | Line 1: | ||
'''Thursday June 17, 2010''' | '''Thursday June 17, 2010''' | ||
| + | |||
| + | [[Image:06.17.2010_B0015+R0040_PCRGel.png|thumb|400px|Jeremy's gel electrophoresis of the colonies resulting from the construction of B0015 (double terminators) and R0040 (TetR repressible promoter)]] | ||
Dev, Chris, Jeremy: Today, Jeremy restreaked the construct of B0015-R0040, did a PCR on it. He then ran a gel electrophoresis on the five colonies that grew. Colonies 1 and 5 are presumed to have the part desired as observed from the gel picture shown on the right. He also wrote a thank you letter to BioAlberta from which we received $1000 today. Dev helped Emily in her construction of I0500-B0034 through the Quick Ligase procedure. We are using presumed I0500 because we are unsure of the quality of the growth. I0500 has so far, failed to grow consistently. Chris redid the construction of J13002-E0032 to test the YFP from the Registry as well as attempting a plasmid switch of J23032 to a kanamycin resistant plasmid. | Dev, Chris, Jeremy: Today, Jeremy restreaked the construct of B0015-R0040, did a PCR on it. He then ran a gel electrophoresis on the five colonies that grew. Colonies 1 and 5 are presumed to have the part desired as observed from the gel picture shown on the right. He also wrote a thank you letter to BioAlberta from which we received $1000 today. Dev helped Emily in her construction of I0500-B0034 through the Quick Ligase procedure. We are using presumed I0500 because we are unsure of the quality of the growth. I0500 has so far, failed to grow consistently. Chris redid the construction of J13002-E0032 to test the YFP from the Registry as well as attempting a plasmid switch of J23032 to a kanamycin resistant plasmid. | ||
Revision as of 23:48, 17 June 2010
Thursday June 17, 2010
Dev, Chris, Jeremy: Today, Jeremy restreaked the construct of B0015-R0040, did a PCR on it. He then ran a gel electrophoresis on the five colonies that grew. Colonies 1 and 5 are presumed to have the part desired as observed from the gel picture shown on the right. He also wrote a thank you letter to BioAlberta from which we received $1000 today. Dev helped Emily in her construction of I0500-B0034 through the Quick Ligase procedure. We are using presumed I0500 because we are unsure of the quality of the growth. I0500 has so far, failed to grow consistently. Chris redid the construction of J13002-E0032 to test the YFP from the Registry as well as attempting a plasmid switch of J23032 to a kanamycin resistant plasmid.
