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__NOTOC__ ===Week12 Highlights=== We began assembling LacP-RBS-CHS3. We defrosted our -20 and in the process reorganized the -20 and threw out unnecessary DNA. ---- ===8/30/10=== Screened MAGE culture for knockouts. ---- ===8/31/10=== Attempted to assemble LacP-RBS-CHS3 --> accidentally cut CHS3 with P (contains a P site) Reorganized the racks in the -20. Threw out unnecessary/old DNA from our -20. Moved all of our racks from our -20 to another -20 in order to defrost our -20. tqsA Knockouts FOUND (?) in MAGE culture upon screening. ---- ===9/1/10=== Ragan left for home today and left MAGE in our hands. He asked us to test his tsqA knockouts to determine if they were in fact true knockouts. ---- ===9/2/10=== Day Off ---- ===9/3/10=== Reorganized -20 DNA stock. Created racks for individual teammates to stay organized once the school year starts. ----

Return to Week12 8/29/10-9/4/10.