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{{Template:Stanford_Main_Menu_0910}} <div style="width:720px;text-align:justify"> ==General Ideas== '''One Idea to measure [B] / [A]''' (assume [A] ≠ 0) *input A induces expression of GFP mRNA *input B induces expression of GFP sRNA (sRNA that targets the GFP mRNA transcript) *so for a given [A], **if [B] = 0, we should see glowing bacteria **if 0 < [B] < [A], we should see some glow **if [B] > [A], we should see no glow '''Another Idea to measure [B] / [A]''' (assume [A] ≠ 0) *input A induces expression of GFP mRNA and YFP sRNA *input B induces expression of YFP mRNA and GFP sRNA *so for a given [A], **if [B] = 0, we should see GREEN glowing bacteria **if 0 < [B] < [A], we should see some glow that is more GREEN than YELLOW. **if [B] > [A], we should see some glow that is more YELLOW than GREEN. ==References== *here's a good reference: [http://www.springerlink.com/content/x6p4lqn1608267r1/fulltext.pdf Engineering RNA-based circuits]. It discusses different methods of regulation by RNA: **via base-pairing (small RNA) **shape-based (riboswitches) **catalytic (ribozymes) *engineered riboregulators (possible mechanism for us) [http://www.nature.com/nbt/journal/v22/n7/pdf/nbt986.pdf Engineered riboregulators enable post-transcriptional control of gene expression] **Note: apparently does not work well in practice *engineering those aforementioned sRNA's: **vague article [http://www.nature.com/nmeth/journal/v4/n12/full/nmeth1207-986b.html Engineers meet small RNA] **some specifics [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1994261/pdf/pbio.0050229.pdf Quantitative Characteristics of Gene Regulation by Small RNA] </div>

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