"

From 2010.igem.org

You do not have permission to edit this page, for the following reason:

You can view and copy the source of this page:

===Methanol Fixation=== # Rinse slide with ethanol and flame # Rinse slide with PBS and dry with KimWipe # Apply cell sample to microscope slide and let air dry # Submerge in -20C absolute methanol for 5-10 min # Wash 3 times with 1X TBS (submerge in 1X TBS for 5 minutes per wash) #*'''Do not let cells dry for the rest of the procedure''' ===Staining=== # Make 1:500 dilution of chitin probe in TBS (50ul of probe is used per slide, [49.9ul of 1X TBS]:[0.1ul of chitin probe] --> 1 slide) # Apply 50ul to the slide containing the sample # Allow stain to incubate overnight at RT in a dark room #* ''To prevent stain from evaporating overnight'': Use a large glass petri dish. Soak a paper towel with PBS and place it at the bottom of the petri dish. Use 2 toothpicks to support the slide above the paper towel. This prevents any wicking action from occurring and pulling the stain off the slide. # Wash 3 times with 1X TBS (submerge in 1X TBS for 5 minutes per wash) # Place a cover slide over sample. Image sample using fluorescent microscope Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]] [[Category:Protocol]]

Return to Team:Northwestern/Protocol/Rhodamine-Conjugated Chitin Probe.