Team:INSA-Lyon/Protocols/Measure of osmotic pressure influence

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<h3> Protocols </h3>
<br><p>Choose a protocol to read its description : 
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<h5 style="text-indent:20px">Measure of osmotic pressure influence</h5>
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Preparation of different media : 0M - 0,01M - 0,05M - 0,1M – 0,5M
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	<li>x (µL)  Sucrose 1M </li>
	<li>2,5mL media M63</li>
	<li>50µL of glucose</li>
	<li>Complet with steril water until 5mL</li>
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Incubate during 24 h at the temperature 28°C and the shaking speed of 100rpm <br/><br/>
Measure of the fluorescence : input wavelenght 490 nm, output one 510 nm.<br/><br/>
Measure of the biomass(absorbance 600 nm): Dilute 250 µL of culture in 750 µL of water.<br/>

<i>Make the blank with 0,5mL of media M63 and 0,5mL of steril water</i></li>

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