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'''Transformation to ''E. coli''''' *Add 10 µL of ligate to 100 µL competent cells *Put cells for 30 minutes on ice *Heat shock the cells for 45 seconds at 42 °C *Put cells on ice for 2 minutes *Add 400 µL LB and grow for 1 hour at 37 °C *Plate 100 µL of cells on LB agar (2%) plates

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