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{{Team:Newcastle/mainbanner}} =Growing an Overnight Culture= ==Materials Required== * LB Broth * Colonies of bacteria * Flame loop * Universal tube * Pipette ==Procedures== # 5ml of LB broth is pipetted into the universal tube. # The flame loop is used to pick a colony. # The colony is then mixed into the LB Broth in the universal tube. # The culture is left overnight at 37°C (''E.coli'', ''B.subtilis'' lower temp) '''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' {{Team:Newcastle/footer}}

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