Week of 6/14

==June 14th==

*Flasks mini prepped
    -J37033 (LuxR + RBS)
    -C0261 (LuxI + RBS)
    -R0061 (Lux Promoter)
    -J23116 (Constitutive Promoter)
    -R0063 (Lux Promoter)
    -K145150 (Lux Promoter)
    -K091146 (Lux Promoter)
    -K274100 (Pigment)
    -J23108 (Constitutive Promoter)

*All of the mini preps were digested with the following:
    -J37033 (XP)
    -C0261 (XP)
    -R0061 (ES)
    -J23116 (ES)
    -R0063 (ES)
    -K145150 (ES)
    -K091146 (ES)
    -K274100 (ES)
    -J23108 (ES)

*We ran the digest as usual: 1 hour in the water bath, 20 minutes heat kill. We did not phosphotase any of these as you can see all of these parts appear on the gel.

*The order was Ladder, J23108, K274100, K091146, K145150, R0063, J23116, R0061, C0261, and J37033.
 



<center>[[Image:6-14-10.jpg]]</center>



*This gel came out well with the exception of K145150.

*We then cut out each available band, purified, ligated, and finally transformed.


<center>[[Image:6-15-10.jpg]]</center>


<center>[[Image:6-16-10.jpg]]</center>


<center>[[Image:6-17-10.jpg]]</center>






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