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__NOTOC__ ==October 17 (Mon)== # To liquid culture. #*95, 105. #* Pick up different colony. # PCR of Cel5B. # Ligation. #* 082: 053, 1-23L(vector) #* 091: 009, 1-23L(vector) #* 094: 007, 1-23L(vector) #* 095(C): 2-4A(upstream), 072(downstream), 1-3A(vector) #* 105(K): 2-4A(upstream), 076(downstream), 1-5A(vector) #* 122(A): 129(upstream), 102(downstream), 1-1C(vector) #* 123(C): 2-4A(upstream), 102(downstream), 1-3A(vector) #* 128: 004(upstream), 086(downstream), 1-1C(vector) #* 129: 005(upstream), 086(downstream), 1-3A(vector) # Restriction digest of gel eluted Fcex1, 035 with <i>Eco</i>RI, <i>Spe</i>I. # Ligation #* 079 and 039. # Transformation of 082(A,K),091(A,K),094(A,K),095(C),100(C),105(K),122(A),123(C),128(A),129(C). # Miniprep of 047, 047-2, 081, 082, 083, 092, 101, 106, 108, 036, 039. # Restriction digest of 047,047-2, 092, 108, 039 with <i>Eco</i>RI, <i>Spe</i>I, 081, 082, 083, 101, 036 with <i>Xba</i>I, <i>Pst</i>I, 100 with <i>Eco</i>RI, <i>Pst</i>I. # To liquid culture #* 031-2, no-name1,2, 2-4A, BYB5013. # Gel Electrophoresis #* 081 -> X #* 082 -> OK #* 083 -> OK #* 092 -> X #* 101 -> X #* 108 -> X #* 036 -> OK #* 039 -> X #* 035 -> OK #* 051 -> X # Ligation #* 085(A,K): Fcex(upstream), 1-23L(vector) #* 092(A,K): 011, 1-23L(vector) #* 111(C): 010(upstream), 082(downstream), 1-3A(vector) #* 112(C): 006(upstream), 082(downstream), 1-3A(vector) #* 113(C): 046(upstream), 082(downstream), 1-3A(vector) #* 114(A): 004(upstream), 083(downstream), 1-1C(vector) #* 115(A): 005(upstream), 083(downstream), 1-1C(vector) #* 079-2(K): 079, 1-5A(vector) #* 100①: 047(upstream), 1-23L(downstream), 1-3A(vector) #* 100②: 039 and 079 #* 100-2(A): 039(upstream), 079(downstream), 1-1C(vector) #* 047(A): 039(upstream), 036(downstream), 1-1C(vector) # PCR of Cel5B [[Team:Osaka/Notebook|Back to Notebook]]

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