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<li><a href="https://2010.igem.org/Team:Chiba/System_2/Testing_individual_parts"><span>Testing</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Construction_Process"><span>Construction</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem"><span>Evaluation</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Result"><span>Result</span></a></li>
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Remarks"><span>Remarks</span></a></li>
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<br><br><br><br><br><br>
<font size=6>System 2 :</font>
===<font size="5">Abstract</font>===
-----
LuxR is AHL-dependent activator.LuxR-AHL complex binds lux box, 20-bp sequence centered at position -42.5 from starting site and activates transcription.However lux box is inserted between -35 and -10,LuxR functions as AHL-inverter (Plux inv). Plux inv is resistered in Biobrick number R0061.We've prepared Plux inv-GFP and characterized about it.<br><br><br>

===<font size="5">Experiments</font>===
-----

===1.Preparing Plux inv-GFP===
1．Plux inv－GFPの作製
クローニングのprocessをFig. 1に示す。作製したPlux inv-GFPを形質転換した株は緑色蛍光を示した。また，sequenceも正しいことを確認した。
Preparing Plux inv-GFP process is shown in '''Fig. 1'''.

[[Image:Plux inv-GFP.png|none|frame|right|'''Fig. 1''' 　Evaluation of LuxR Inverter]]

===2.Characterization Plux inv-GFP===
Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。コロニーをつついてLB液体培地(AHL0および1000 nM)で12 h培養し,
スピンダウンしてペレットを観察した。
[[Image:lux inverter function.png|frame|center|Fig. 2 assay protocol]]

<br><br><br>
===<font size="5">Results</font>===
-----
[[Image:Plux inv results.png|none|right|Fig. 3 ]]

<br><br><br>

===<font size="5">Reference</font>===
-----
#Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
#Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)