Team:NYMU-Taipei

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<font size=5>'''SpeedyBac'''</font><br>
<br>
*'''<font size=3>Goal</font>:'''<br>
Provide a faster assay system for exploring the design rules of synthetic biology.
*'''<font size=3>Why do we want to do that?</font><br>
There are more and more parts in partsregistry. Every igem teams try to build one or more circuits from partsregistry. But what are the design rules in a big circuit system. We are very interesting what the more detail rules are in the central dogma; especially between the mRNA translations to protein folding. Previously, we know about the circuits we done are work or none by the expression of reporter genes. But now we want to quantitative description of gene expression in both space and time. For the above reasons, we must to be speed up the experiment for researching the more rules. <br>
*'''<font size=3>Specific aims:</font>'''<br>
**Quantitative description of gene expression in both space and time.
**Specific insight into the flow of genetic  information.
**Speedy ways to report and stop gene expression.
*'''<font size=3>Our design:</font>'''<br>
For achieve our specific aim, we design a novel reporting assay [[Team:NYMU-Taipei/Project/Speedy reporter|(Speedy reporter)]] for quickly detect and measure the mRNA location and quantity, it can be also use for protein detection. And we design a novel switch [[Team:NYMU-Taipei/Project/Speedy switch|(Speedy switch)]] for control the translation in gene expression. We have also designed a faster degradation system [[Team:NYMU-Taipei/Project/Speedy protein degrader |(Speedy protein degrader)]]; it allows us to regulate the degradation time for study the mRNA without the interference from translation and quickly stop the gene expression.<br>
'''The parts our project is made up of''':<br>
* [[Team:NYMU-Taipei/Project/Speedy switch | Speedy switch]]
** Faster production of protein by inducing the translation of pre-transcribed RNA molecules. 
* [[Team:NYMU-Taipei/Project/Speedy reporter| Speedy reporter]]
** Using mRNA aptamers and split GFP-eIF4A reporter systems to show promoter activity faster.
* [[Team:NYMU-Taipei/Project/Speedy protein degrader | Speedy protein degrader]]
** Fast, specific, and constitutive proteolysis achieved by engineering fluorescent proteins with LVA 
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