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==October 5th== *Digests: -R1062+RFP (SP) -R0061+RFP (SP) *Ligations: -R1062+RFP -->J23100+LuxR (B) -->J23108+LuxR (B) -->J23116+LuxR (B) -R0063+RFP -->J23100+LuxR (B) -->J23108+LuxR (B) -->J23116+LuxR (B) ==October 6th== *Colony PCR: 1. R1062+RFP + J23100+LuxR 2. R1062+RFP + J23108+LuxR 3. R1062+RFP + J23116+LuxR 4. R0063+RFP + J23100+LuxR 5. R0063+RFP + J23108+LuxR 6. R0063+RFP + J23116+LuxR They were then run on a gel after being PCRd. *Gel Order: [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]

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