Protocol/4

{{Team:Alberta/Head}}

{{Team:Alberta/navbar|project=selected}}

{{Team:Alberta/beginLeftSideBar}}
{{Team:Alberta/endLeftSideBar}}

{{Team:Alberta/beginRightSideBar}}
{{Team:Alberta/endRightSideBar}}

{{Team:Alberta/beginMainContent}}

Protocol 4: Plasmid Miniprep


What you will need:

* Overnight culture
* Qiagen plasmid miniprep kit 

Procedure:

* Transfer 1.5 ml of culture to Eppendorf.
* Spin down at 13 000 rpm. 
* Discard supernatant and follow procedure on p. 22 of [http://www.qiagen.com/literature/render.aspx?id=370 QIAprep Miniprep Handbook].
* Determine concentration of purified plasmid with nanodrop

==Notes==
* Low speed pellet is easier to resuspend.
* To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the holes, so it makes a "quacking" sound. It's pretty fun and works pretty good. Vortex after too.
* Step 2 in Qiagen, the alkaline lysis with buffer P2, should be brief to avoid damaging DNA.

[[Team:Alberta/Notebook/protocols| Back]]
{{Team:Alberta/endMainContent}}