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==August 10th & 11th== *Grew up Lux Promoters (R0061, R0063, K091146, K145150, K091107, R1062) *Made SOB media *Minipreps: - K091107, K091146, R0063, R1062, K145150 - R0061 did not grow - LP(RFP+RBS) + 2 minipreps of RFP+RBS *Digests: - Lux promoters with S+P - RFP+RBS with E+P and X+P(Phosphatased) - Digested K3+LP with E+P, K3 was phosphatased *Gel order: -Ladder -K091107 -R0063 -R1062 -K091146 -K145150 -RFP+RBS (EP) -RFP+RBS (XP) -K3 -LP *Ligations: - RFP+RBS + Lux promoters - K3 + LP - K3 + RFP+RBS [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]

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