"

From 2010.igem.org

You do not have permission to edit this page, for the following reason:

You can view and copy the source of this page:

{{:Team:St_Andrews/defaulttemplate}} ==Protocols== We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials. ===Cell Culture=== OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture] ===Transformation=== [http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli] [http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.] ===Restriction Digest=== Promega Enzymes NEB Biobrick Assembly Kit (Gingko Bioworks) ===Ligation=== NEB Biobrick Assembly Kit (Gingko Bioworks) ===PCR=== OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR] NEB Taq Finnzymes Phusion ===DNA Extraction=== [http://www.qiagen.com/products/plasmid/qiaprepminiprepsystem/qiaprepspinminiprepkit.aspx#Tabs=t2 Quiagen Miniprep] Isopropanol Extraction of small DNA fragments [Team:St_Andrews/project/laboratory/protocols/isopropanol From another lab in St Andrews] ===Construction of BioBricks=== Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit) OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy) Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself) OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]

Return to Team:St Andrews/project/laboratory/protocols.