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__NOTOC__ ===Week12 Summary=== We began reorganizing our lab space and equipment in preparation for a fall quarter class that would be utilizing the majority of the lab. We attempted standard assembly of LacP-RBS-CHS3 because the presence of a P site in CHS3 prevented 3A assembly. ---- ===9/6/10=== Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room. ===9/7/10=== ******** ---- ===9/8/10=== ******** ---- ===9/9/10=== Assembled LacP-RBS1-CHS3 in a Chlor backbone plasmid using standard assembly methods Cut LacP-RBS1 using S Cut CHS3 using X and S (50% chance of inserting backwards --> must select for this after) ---- ===9/10/10=== Inoculated 20 LacP-RBS-CHS3 cells. Assembled CP-LacPI-RFP parts into Tet backbone. ----

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