Team:Tsinghua/Notebook/24 September 2010
From 2010.igem.org
Module Ⅱ, group 4
Transform the trans5α with OmpA and CipC, respectively.
Transform the trans5α with pET21b.
Inoculation of OmpA、CipC、pET21b, 37℃,12h
Plasmid abstraction (OmpA、CipC、pET21b)
Measuring the concentration of the three kinds of plasmids.
result
The concentration of OmpA is 125.1ng/ul;
The concentration of CBD is 117.3ng/ul;
The concentration of OmpA is 269.4ng/ul.