Aleksandra
Touchdown PCR of the 13 minipreps from yesterday and also of 6 other constructs (elongation time 6 mins), then gel electrophoresis.

Gel 1 (PCR)

Minipreps from 31/08

• 1 Pms/wTet II
• 2 Pms/mTet III
• 3 Psm/mTet III
• 4 Psw/mTet III
• 5 Pss/wTet II
• 6 Psw/wTet II

Minipreps from yesterday (04/05)

• 7,8 Pms/mTet/LuxI, 2 cultures
• 9,10 Pss/wTet/LuxI, 2 cultures
• 11,12 Psm/sTet/LuxI, 2 cultures
• 13,14 Psm/wTetIII/I13507/pLux/GFP, 2 cultures
• 15,16 Psm/wTetII/I13507/pLux/GFP, 2 cultures
• Ladder 1 kb

<a href="/wiki/Image:PCR0509.jpg" class="image" title="Image:PCR0509.jpg"><img alt="Image:PCR0509.jpg" src="/images/thumb/4/49/PCR0509.jpg/687px-PCR0509.jpg.png" width="687" height="386" border="0" /></a>

Gel 2 (PCR)

Minipreps from yesterday (04/05)

• 17, 18 Pss/sLac/LuxI III, 2 cultures
• 19 Pms/sTet/LuxI

Digestion from today(see further)

• 19 Pms/sTet/LuxI

<a href="/wiki/Image:PCR0509_2.jpg" class="image" title="Image:PCR0509_2.jpg"><img alt="Image:PCR0509_2.jpg" src="/images/thumb/0/0c/PCR0509_2.jpg/335px-PCR0509_2.jpg.png" width="335" height="400" border="0" /></a>

→ Colonies 7, 9, 10, 17, 18, 19 seem to be of a right size

Restriction digest and gel electrophoresis of yesterday's minipreps with E+P

Gel 1

• 1 attP/PmsRFP, col 1
• Ladder 1kb
• 2 attP/PmsRFP, col 2
• -
• 3 attP/PsmRFP, col 1
• -
• 4 attP/PsmRFP, col 2
• -
• 5 attP/PssRFP, col 1
• -
• 6 attP/PssRFP, col 2
• -
• 7 Pms/mTet/LuxI, col 1
• -
• 8 Pms/mTet/LuxI, col 2
• -
• 9 Pss/wTet/LuxI, col 1

<a href="/wiki/Image:Gel0509_2uncut.jpg" class="image" title="Image:gel0509_2uncut.jpg"><img alt="Image:gel0509_2uncut.jpg" src="/images/thumb/0/0d/Gel0509_2uncut.jpg/682px-Gel0509_2uncut.jpg.png" width="682" height="408" border="0" /></a>

Gel 2

• 10 Pss/wTet/LuxI, col 2
• -
• 11 Psm/sTet/LuxI, col 1
• -
• 12 Psm/sTet/LuxI, col 2
• -
• 13 Psm/wTetIII/I13507/pLux/GFP, col 1
• -
• 14 Psm/wTetIII/I13507/pLux/GFP, col 1
• -
• 15 Psm/wTetII/I13507/pLux/GFP, col 1
• -
• 16 Psm/wTetII/I13507/pLux/GFP, col 2
• -
• 17 Pss/sLac/LuxI III, col 1
• -
• 18 Pss/sLac/LuxI III, col 2

<a href="/wiki/Image:Gel0509_1uncut.jpg" class="image" title="Image:gel0509_1uncut.jpg"><img alt="Image:gel0509_1uncut.jpg" src="/images/thumb/5/5b/Gel0509_1uncut.jpg/684px-Gel0509_1uncut.jpg.png" width="684" height="399" border="0" /></a>
<a href="/wiki/Image:Gel0509_1cut.jpg" class="image" title="Image:gel0509_1cut.jpg"><img alt="Image:gel0509_1cut.jpg" src="/images/thumb/0/01/Gel0509_1cut.jpg/690px-Gel0509_1cut.jpg.png" width="690" height="388" border="0" /></a>

• 19 Pms/sTet/LuxI (on the PCR gel, see above)

→ Digestions 1, 2, 7, 9, 10, 17, 18, 19 seem to be of a right size