"

From 2010.igem.org

05.08.2010

• digest of cheB-A, cheB-C, cheR-A, cheR-C, cyFP-A, cyFP-C, tetR-A with Aar1
• gel of digest, see results
• gel extraction of cheB-A, cheB-C, cyFP-A and tetR-A
• miniprep of PIF3-A, PIF3-C, PhyB-C, Trig-A

06.08.2010

• inoculation of cheR-A, cheR-C, cyFP-A and cyFP-C. Since the digest of 05.08.10 didn't work out very nicely we'll start out from the colonies again
• transformation MreB-C and plating out