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From 2010.igem.org

9/3/10

Today I’m finishing making the LB/Agar from yesterday. 1) I took the 2 Elenmyier Flasks from hot water bath and melting in microwave. 2) Microwaving on beverage setting on 8 oz. setting until liquid. 3) then placed into autoclave on liquid setting/ 121 C 15 min 4) the placed into fridge until needed. Now I’m make up 200 mL of agarous Gel 1) I grabbed a 250 mL grad cylinder and a 100 grad cylinder, 2) 250 black capped bottles, 50X TAE Buffer Stock, BR – 1000 micropippettor, Tips and Agar Biograde, weighed out 1.0003 g & 1.0013 grams of agar placed one into each bottle next added 2 mL of 50X TAE stock into each bottle then filled with 98 mL 18 ohm H2O 3) next placed both bottles on a hot plate set at 400 degrees to melt 4) After bringing to a low boil I dropped temp to 250 degrees.