INSA-Lyon/13 September 2010
From 2010.igem.org
September 13th
Start of 6 liquid cultures of PHL1273 : waterbath 28°C, 3 tubes at 160 rpm and 3 tubes at 80 rpm
DNA extraction of 18A, I0500 and 14K
Digestion :
- 8C/14K, backbone tet : E+P
- 1F, 4O,18A, 14 (pha C from IGEM plates) : E+S
- 24A, 10L, L3 : X+P
Ligation in backbone tet of : 1F and 24A , 4O and 10L
Resuspension of the DNA synthese we just received from Mr Gene : Phasin Intein
Second purification of the clone from the ligation of 12/13 (from the 07/09)
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