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From 2010.igem.org

Contents 1 October 5th 2 October 6th 3 October 7th 4 October 15 to 17

October 5th

• Digests:

-R1062+RFP (SP)
-R0061+RFP (SP)

• Ligations:

-R1062+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)

October 6th

We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample.

• Colony PCR:

1. R1062+RFP + J23100+LuxR
2. R1062+RFP + J23108+LuxR
3. R1062+RFP + J23116+LuxR
4. R0063+RFP + J23100+LuxR
5. R0063+RFP + J23108+LuxR
6. R0063+RFP + J23116+LuxR

They were then run on a gel after being PCR'd.

• Gel Order:

- 1.a,b,c
- 2.a,b,c
- 3.a,b,c
- 4.a,b,c
- 5.a,b,c
- 6.a,b,c

• Grown In Flasks:

-1a (R1062+RFP+J23100+LuxR)
-2a (R1062+RFP+J23108+LuxR)
-3a (R1062+RFP+J23116+LuxR)
-4a (R0063+RFP+J23100+LuxR)
-5a (R0063+RFP+J23108+LuxR)
-6a (R0063+RFP+J23116+LuxR)

October 7th

Referencing the Notation used on October 6th.

• Minipreps:

-1a
-2a
-3a
-4a
-5a
-6a

• Lab Work

-Made Stock Solutions of the Minipreps
-Made SOB Media 
-Made Competent Cells
-Poured Agar Plates
-Sent Samples to Sequencing Center

We began to anticipate the Jamboree at this point and were diligently working to finish on time.

Return to Notebook

October 15 to 17

Tecan data collected: P6 promoter

D5 promoter

J23113 promoter

K091107 Lux promoter with J23108 expressing LuxR

K091107 Lux promoter with J23116 expressing LuxR

K091146 Lux promoter with J23108 expressing LuxR

K091146 Lux promoter with J23116 expressing LuxR