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User:VolkerMorath
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The insertion of sequences for functional peptides into the viral capsid is a well established and characterized possibility to retarget and modify the viral vectors. Over the last decade many different locations in the viral sequence have been evaluated for their quality to insert small peptides. We decided to use the well etablished 587 and the newly identifyed 453 integration site for our Virus Construction Kit.<br> | The insertion of sequences for functional peptides into the viral capsid is a well established and characterized possibility to retarget and modify the viral vectors. Over the last decade many different locations in the viral sequence have been evaluated for their quality to insert small peptides. We decided to use the well etablished 587 and the newly identifyed 453 integration site for our Virus Construction Kit.<br> | ||
For reasony of flexibility and usability we decided not to use single cutting restriction sites in stead of PCR-technology. This approach provids the advantage that all capsid coding constructs can be modifyed using ViralBricks in a single cloning step but also beared the risk to harm the functionality of the viral gene sequence. | For reasony of flexibility and usability we decided not to use single cutting restriction sites in stead of PCR-technology. This approach provids the advantage that all capsid coding constructs can be modifyed using ViralBricks in a single cloning step but also beared the risk to harm the functionality of the viral gene sequence. | ||
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<div style="float:right; width:965px; height:auto; "><img src="http://partsregistry.org/wiki/images/5/5a/Freiburg10_ViralBrick_compatible_Capsid_coding_sequence.png | <div style="float:right; width:965px; height:auto; "><img src="http://partsregistry.org/wiki/images/5/5a/Freiburg10_ViralBrick_compatible_Capsid_coding_sequence.png | ||
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Revision as of 20:14, 15 October 2010
=> Vector Plasmid
=> Capsid Coding Parts
=> Miscellaneous System parts
=> Pictures
ViralBrick compatible version of the capsid coding sequence
The insertion of sequences for functional peptides into the viral capsid is a well established and characterized possibility to retarget and modify the viral vectors. Over the last decade many different locations in the viral sequence have been evaluated for their quality to insert small peptides. We decided to use the well etablished 587 and the newly identifyed 453 integration site for our Virus Construction Kit.
For reasony of flexibility and usability we decided not to use single cutting restriction sites in stead of PCR-technology. This approach provids the advantage that all capsid coding constructs can be modifyed using ViralBricks in a single cloning step but also beared the risk to harm the functionality of the viral gene sequence.
For reasony of flexibility and usability we decided not to use single cutting restriction sites in stead of PCR-technology. This approach provids the advantage that all capsid coding constructs can be modifyed using ViralBricks in a single cloning step but also beared the risk to harm the functionality of the viral gene sequence.
