UTDallas/7 October 2010
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===October 7, 2010 === | ===October 7, 2010 === | ||
*Performed the large scale titration of PyeaR+GFP with 0M, 10uM, 50uM, 100uM, 500uM, 1mM, and 10mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite. | *Performed the large scale titration of PyeaR+GFP with 0M, 10uM, 50uM, 100uM, 500uM, 1mM, and 10mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite. | ||
- | *Results show that PyeaR is more sensitive to nitrates then nitrites (just like it says on the parts registry). | + | **Results show that PyeaR is more sensitive to nitrates then nitrites (just like it says on the parts registry). |
- | *They also show that there is an increase in intensity when the concentration of the nitrate/nitrite is increased. | + | **They also show that there is an increase in intensity when the concentration of the nitrate/nitrite is increased. |
*Miniprepped, digested, and ran gel electrophoresis on Pu. Did not get a good band, therefore incubated more for tomorrow. | *Miniprepped, digested, and ran gel electrophoresis on Pu. Did not get a good band, therefore incubated more for tomorrow. | ||
[[Image:10-7.JPG | 500px | center ]] | [[Image:10-7.JPG | 500px | center ]] |
Latest revision as of 21:49, 21 October 2010
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October 7, 2010
- Performed the large scale titration of PyeaR+GFP with 0M, 10uM, 50uM, 100uM, 500uM, 1mM, and 10mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite.
- Results show that PyeaR is more sensitive to nitrates then nitrites (just like it says on the parts registry).
- They also show that there is an increase in intensity when the concentration of the nitrate/nitrite is increased.
- Miniprepped, digested, and ran gel electrophoresis on Pu. Did not get a good band, therefore incubated more for tomorrow.