UTDallas/30 September 2010
Notebook
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September 30, 2010
- Incubate the PyeaR+reporters in 0, 10uM, 100uM, and 1mM potassium nitrate solutions, then observed under the fluorescence microscope.
- The intensity of the fluorescence increased with increasing molarity of nitrate solution.
- Plan to do a larger scale titration test to get better data.
- Miniprepped, digested, ran gel electrophoresis, and gel purified Pu.
- Made LB carb. broth and more carb. plates.
Alkane Sensor
- Had colonies from yesterday's transformation
- Incubated transformants for miniprepping