UTDallas/29 September 2010

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  • Made glycerol stock and miniprepped PyeaR+GFP and PyeaR+RFP.
  • Incubated the PyeaR+reporters in 250uM Potassium Nitrate solution, and then observed under the fluorescence microscope. *All but one had fluorescence, will do more precise testing another day.
  • Incubated Pu, PyeaR+GFP, and PyeaR+RFP overnight.