UTDallas/28 September 2010
From 2010.igem.org
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*Probably a problem with the Pu+GFP part, therefore we will try to make our own Pu+GFP part. | *Probably a problem with the Pu+GFP part, therefore we will try to make our own Pu+GFP part. | ||
*Incubated more colonies from the PyeaR+reporters for glycerol stock, miniprepping, and an initial look at it with nitrates. | *Incubated more colonies from the PyeaR+reporters for glycerol stock, miniprepping, and an initial look at it with nitrates. | ||
+ | Alkane Sensor | ||
+ | *Midiprep of pSB1C3 | ||
+ | *Digestion of pSB1C3 |
Latest revision as of 23:56, 19 October 2010
Notebook
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September 28, 2010
- Tested Pu+GFP+Pr+XylR with xylene. Again had leakage, all had approximately the same intensity.
- Probably a problem with the Pu+GFP part, therefore we will try to make our own Pu+GFP part.
- Incubated more colonies from the PyeaR+reporters for glycerol stock, miniprepping, and an initial look at it with nitrates.
Alkane Sensor
- Midiprep of pSB1C3
- Digestion of pSB1C3