UTDallas/24 September 2010
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(New page: *We digested PyeaR with the new SpeI and PstI-HF that we received in the mail. *We then ran a gel (see picture) which gave the correct band, we only took the bottom portion for gel purific...)
Newer edit →
(New page: *We digested PyeaR with the new SpeI and PstI-HF that we received in the mail. *We then ran a gel (see picture) which gave the correct band, we only took the bottom portion for gel purific...)
Newer edit →
Revision as of 11:48, 28 September 2010
- We digested PyeaR with the new SpeI and PstI-HF that we received in the mail.
- We then ran a gel (see picture) which gave the correct band, we only took the bottom portion for gel purification since that is the part that is correct.
- We then ligated PyeaR to GFP, RFP, and orange overnight at 14C.