UTDallas/22 October 2010
Notebook
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October 22, 2010
- Incubated colonies from PyeaR+reporter (pSB1C3) transformations
- Made Chlora plates
- Transformation results showed that ligations did not work
- Digested GFP and conducted gel electrophoresis, gel purification, Dephosphorylation, and PCR purification
- Ligation and transformation with pAlkB + GFP
- Incubated colonies from pAlkB + GFP from previous day
- Alkane sensor
- miniprep overnight grown colonies.
- did restriction digestion using XBaI and PstI-HF
- Ran a 2% agarose gel. Nothing seems to be positive
- Again transformed the part E0240 into DH5alpha to do midiprep and get more as the previous one didn't work.