UTDallas/22 October 2010

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October 22, 2010

  • Incubated colonies from PyeaR+reporter (pSB1C3) transformations
  • Made Chlora plates
  • Transformation results showed that ligations did not work
  • Digested GFP and conducted gel electrophoresis, gel purification, Dephosphorylation, and PCR purification
  • Ligation and transformation with pAlkB + GFP
  • Incubated colonies from pAlkB + GFP from previous day