UTDallas/22 October 2010

From 2010.igem.org

(Difference between revisions)
(October 22, 2010)
(October 22, 2010)
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*Ligation and transformation with pAlkB + GFP
*Ligation and transformation with pAlkB + GFP
*Incubated colonies from pAlkB + GFP from previous day
*Incubated colonies from pAlkB + GFP from previous day
-
 
[[Image:10-22.JPG | 500px | center]]
[[Image:10-22.JPG | 500px | center]]
 +
;Alkane sensor
 +
*miniprep overnight grown colonies.
 +
*did restriction digestion using XBaI and PstI-HF
 +
*Ran a 2% agarose gel. Nothing seems to be positive

Revision as of 20:30, 27 October 2010


Notebook

June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


October 22, 2010

  • Incubated colonies from PyeaR+reporter (pSB1C3) transformations
  • Made Chlora plates
  • Transformation results showed that ligations did not work
  • Digested GFP and conducted gel electrophoresis, gel purification, Dephosphorylation, and PCR purification
  • Ligation and transformation with pAlkB + GFP
  • Incubated colonies from pAlkB + GFP from previous day
10-22.JPG
Alkane sensor
  • miniprep overnight grown colonies.
  • did restriction digestion using XBaI and PstI-HF
  • Ran a 2% agarose gel. Nothing seems to be positive