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UTDallas/22 October 2010
From 2010.igem.org
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===October 22, 2010 === | ===October 22, 2010 === | ||
*Incubated colonies from PyeaR+reporter (pSB1C3) transformations | *Incubated colonies from PyeaR+reporter (pSB1C3) transformations | ||
| + | *Made Chlora plates | ||
| + | *Transformation results showed that ligations did not work | ||
| + | *Digested GFP and conducted gel electrophoresis, gel purification, Dephosphorylation, and PCR purification | ||
| + | *Ligation and transformation with pAlkB + GFP | ||
| + | *Incubated colonies from pAlkB + GFP from previous day | ||
Revision as of 18:25, 24 October 2010
October 22, 2010
- Incubated colonies from PyeaR+reporter (pSB1C3) transformations
- Made Chlora plates
- Transformation results showed that ligations did not work
- Digested GFP and conducted gel electrophoresis, gel purification, Dephosphorylation, and PCR purification
- Ligation and transformation with pAlkB + GFP
- Incubated colonies from pAlkB + GFP from previous day
