UTDallas/21 October 2010

From 2010.igem.org

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(October 20, 2010)
(October 21, 2010)
 
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===October 21, 2010 ===
===October 21, 2010 ===
*Made carb. plates
*Made carb. plates
-
*Incubated colonies from the transformed PyeaR parts
+
*The transformations of the ligated parts worked. Everything but the negative controls had colonies.
*Transformed the ligated PalkB+GFP
*Transformed the ligated PalkB+GFP
*Performed titration of PyeaR+RFP with 0M, 10uM, 500uM, 10mM, and 100mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite
*Performed titration of PyeaR+RFP with 0M, 10uM, 500uM, 10mM, and 100mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite
 +
;Alkane sensor
 +
*-ve control had colonies in it which is not a good sign.
 +
*However still screened 26 colonied from the other plates and two from the -ve ctrl to see if in case anything is positive.

Latest revision as of 20:26, 27 October 2010


Notebook

June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


October 21, 2010

  • Made carb. plates
  • The transformations of the ligated parts worked. Everything but the negative controls had colonies.
  • Transformed the ligated PalkB+GFP
  • Performed titration of PyeaR+RFP with 0M, 10uM, 500uM, 10mM, and 100mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite
Alkane sensor
  • -ve control had colonies in it which is not a good sign.
  • However still screened 26 colonied from the other plates and two from the -ve ctrl to see if in case anything is positive.