UTDallas/17 September 2010
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*Diluted down (1:50 ratio) the Pu+GFP+Pr+XylR to incubate until they had an OD of 0.06-0.1 on our nanodrop. | *Diluted down (1:50 ratio) the Pu+GFP+Pr+XylR to incubate until they had an OD of 0.06-0.1 on our nanodrop. | ||
*Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fluorescence microscope. Did not see a significant difference between the ones incubated and the ones not incubated. Will try again later. | *Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fluorescence microscope. Did not see a significant difference between the ones incubated and the ones not incubated. Will try again later. | ||
- | *Digested the parts miniprepped yesterday and ran gel electrophoresis | + | *Digested the parts miniprepped yesterday and ran gel electrophoresis and then since the bands were correct gel purified. |
[[Image:9-17.JPG| 500 px | center]] | [[Image:9-17.JPG| 500 px | center]] |
Latest revision as of 17:08, 27 September 2010
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September 17, 2010
- Diluted down (1:50 ratio) the Pu+GFP+Pr+XylR to incubate until they had an OD of 0.06-0.1 on our nanodrop.
- Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fluorescence microscope. Did not see a significant difference between the ones incubated and the ones not incubated. Will try again later.
- Digested the parts miniprepped yesterday and ran gel electrophoresis and then since the bands were correct gel purified.