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UTDallas/17 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: *Diluted down (1:50 ratio) the Pu+GFP+Pr+XylR to incubate until they had an OD of 0.06-0.1 on our nanodrop. *Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fl...) |
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*Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fluorescence microscope. Did not see a significant difference between the ones incubated and the ones not incubated. Will try again later. | *Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fluorescence microscope. Did not see a significant difference between the ones incubated and the ones not incubated. Will try again later. | ||
*Digested the parts miniprepped yesterday and ran gel electrophoresis (see attached photo)and then since the bands were correct gel purified. | *Digested the parts miniprepped yesterday and ran gel electrophoresis (see attached photo)and then since the bands were correct gel purified. | ||
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| + | [[Image:9-17.jpg | 500 px | center]] | ||
Revision as of 22:20, 26 September 2010
- Diluted down (1:50 ratio) the Pu+GFP+Pr+XylR to incubate until they had an OD of 0.06-0.1 on our nanodrop.
- Incubated the Pu+GFP+Pr+XylR with various aromatics, then observed under the fluorescence microscope. Did not see a significant difference between the ones incubated and the ones not incubated. Will try again later.
- Digested the parts miniprepped yesterday and ran gel electrophoresis (see attached photo)and then since the bands were correct gel purified.
