UTDallas/16 October 2010

From 2010.igem.org

(Difference between revisions)
(New page: *Made chloramphenicol plates *Transformed the ligations from yesterday. *I also realized that the ligations will give us the wrong product. We took a random part (BBa_J04450) with the pSB1...)
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*Transformed the ligations from yesterday.
*Transformed the ligations from yesterday.
*I also realized that the ligations will give us the wrong product. We took a random part (BBa_J04450) with the pSB1C3 backbone to digest to get just the backbone, but I did not digest it properly, so the ligations will give us our parts (PyeaR+GFP and PyeaR+RFP) behind LacI+RFP. Therefore I will be digesting and ligating again during the week.
*I also realized that the ligations will give us the wrong product. We took a random part (BBa_J04450) with the pSB1C3 backbone to digest to get just the backbone, but I did not digest it properly, so the ligations will give us our parts (PyeaR+GFP and PyeaR+RFP) behind LacI+RFP. Therefore I will be digesting and ligating again during the week.
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Alkane Sensor
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*Transformed ligation parts from yesterday

Revision as of 23:59, 19 October 2010

  • Made chloramphenicol plates
  • Transformed the ligations from yesterday.
  • I also realized that the ligations will give us the wrong product. We took a random part (BBa_J04450) with the pSB1C3 backbone to digest to get just the backbone, but I did not digest it properly, so the ligations will give us our parts (PyeaR+GFP and PyeaR+RFP) behind LacI+RFP. Therefore I will be digesting and ligating again during the week.

Alkane Sensor

  • Transformed ligation parts from yesterday