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UTDallas/15 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: *the transformations did not work from yesterday, so we decided to redigest the parts to make certain that they were actually cut with both enzymes. *We then ran some gels, both gels had ...) |
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*We also tested the Pu+GFP+Pr+XylR broths for fluorescence again, and the negative control is still as fluorescent as the others. We will try again later. | *We also tested the Pu+GFP+Pr+XylR broths for fluorescence again, and the negative control is still as fluorescent as the others. We will try again later. | ||
*We incubated from glycerol stock most of the parts used today for miniprepping tomorrow. | *We incubated from glycerol stock most of the parts used today for miniprepping tomorrow. | ||
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| + | The gels: | ||
| + | [[Image:9-15.jpg]] | ||
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| + | [[Image:9-15(2).jpg]] | ||
Revision as of 12:01, 21 September 2010
- the transformations did not work from yesterday, so we decided to redigest the parts to make certain that they were actually cut with both enzymes.
- We then ran some gels, both gels had the appropriate results so we gel purified and then ligated.
- We also tested the Pu+GFP+Pr+XylR broths for fluorescence again, and the negative control is still as fluorescent as the others. We will try again later.
- We incubated from glycerol stock most of the parts used today for miniprepping tomorrow.
The gels:
.jpg)
