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Revision as of 12:02, 21 September 2010

• the transformations did not work from yesterday, so we decided to redigest the parts to make certain that they were actually cut with both enzymes.
• We then ran some gels, both gels had the appropriate results so we gel purified and then ligated.
• We also tested the Pu+GFP+Pr+XylR broths for fluorescence again, and the negative control is still as fluorescent as the others. We will try again later.
• We incubated from glycerol stock most of the parts used today for miniprepping tomorrow.

The gels: