UTDallas/15 October 2010
Notebook
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October 15, 2010
- Miniprepped PyeaR+GFP and PyeaR+RFP.
- Digested PyeaR+GFP, PyeaR+RFP, and pSB1C3, then ran a gel electrophoresis and gel purified. All of them were correct and gave good yields.
- Ligated PyeaR+GFP and PyeaR+RFP to pSB1C3, overnight.
Alkane Sensor
- Ligated dephosphorylated pSB1C3 to phosphorylated PalkB