UTDallas/15 October 2010

From 2010.igem.org

(Difference between revisions)
(New page: *Miniprepped PyeaR+GFP and PyeaR+RFP. *Digested PyeaR+GFP, PyeaR+RFP, and pSB1C3, then ran a gel electrophoresis and gel purified. All of them were correct and gave good yields. *Ligated P...)
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[[Image:10-15.JPG | 500px | center]]
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Alkane Sensor
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*Ligated dephosphorylated pSB1C3 to phosphorylated PalkB

Revision as of 23:59, 19 October 2010

  • Miniprepped PyeaR+GFP and PyeaR+RFP.
  • Digested PyeaR+GFP, PyeaR+RFP, and pSB1C3, then ran a gel electrophoresis and gel purified. All of them were correct and gave good yields.
  • Ligated PyeaR+GFP and PyeaR+RFP to pSB1C3, overnight.
10-15.JPG

Alkane Sensor

  • Ligated dephosphorylated pSB1C3 to phosphorylated PalkB