UTDallas/14 September 2010

From 2010.igem.org

• we took the broth incubated overnight split it into four parts, three with either toluene, benzene, and xylene, and the other with nothing.
• We let them incubate for a few hours and then tested for fluorescence.
• All of them were equally fluorescent so we left them to incubate overnight.
• This means that the promoter might leak.
• We also transformed the ligations.