ULB/5 October 2010

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Biobricks construction

PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work.
PCR on ParD and ParE to have those genes outside of a plasmid (as PCR products). Worked. We used a plasmid containing both genes as template.
PCR on FLP, using the sequence we got synthesized as backbone, to add RCF 10 prefix and suffix to the gene. Didn't work.
Digestion of Cm and Kan resistance cassettes with RCF10 enzymes in order to insert them in pSB1C3. Worked.

Module hydrogen

We obtained bacteria strains with two deleted genes: one strain that lacked LdhA and FocA and the other one was missing LdhA and PPC.